Stimulation of Phosphatidylethanolamine Exchange by Castor Bean Cytosol Proteins
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چکیده
منابع مشابه
Stinulation of Phosphatidylethanolamine Exchange by Castor Bean Cytosol
Cytosol proteins prepared from castor bean endosperm (4-day-old) s stimulate the exchange of [IHlpbosphatidylethanolamine between liposomes and mitochondria. The acceleration of the exchange depends on the quantity of cytosol proteins, the time of incubation, and the respective amounts of liposomes and mitochondria. On a per seeding basis, the active proteins are essentiafly located in the endo...
متن کاملPhosphatidylethanolamine synthesis by castor bean endosperm : a base exchange reaction.
A base exchange reaction for synthesis of phosphatidylethanolamine by the endoplasmic reticulum of castor bean (Ricinus comminus L. var Hale) endosperm has been examined. The calculated Michaelis-Menten constant of the enzyme for ethanolamine was 5 micromolar and the optimal pH was 7.8 in the presence of 2 millimolar CaCl(2). l-Serine, N-methylethanolamine and N,N-dimethylethanolamine all reduc...
متن کاملPhosphatidylethanolamine synthesis in castor bean endosperm.
Phosphatidylethanolamine synthesis by CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) from the endoplasmic reticulum of castor bean (Ricinus communis L. var. Hale) endosperm was characterized. The Michaelis-Menten constant of the enzyme for CDP-ethanolamine was approximately 8.0 micromolar. The pH optimum was 6.5 and a divalent cation was an absolute requirement ...
متن کاملPhospholipid synthesis and exchange in castor bean endosperm homogenates.
Crude organelle preparations from castor bean (Ricinus communis L.) endosperm rapidly incorporate CDP-((14)C)choline and CDP-((14)C)-ethanolamine into phosphatidylcholine and phosphatidylethanolamine, respectively. Separation of organelles by sucrose density gradient centrifugation following incubation with these substrates demonstrated that most of the (14)C phospholipids thus formed were pres...
متن کاملAlbumin storage proteins in the protein bodies of castor bean.
Of the total protein in the protein bodies of castor bean (Ricinus communis L.), approximately 40% is represented by a group of closely related albumins localized in the matrix of the organelle. This group of albumins has a sedimentation value of 2S and is resolved into several proteins of molecular weight around 12,000 daltons by sodium dodecyl sulfate-acrylamide gel electrophoresis. It has a ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1980
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.65.2.355